Search Results

Abstract : Citrus peel is a worthy bioactive compound source to develop. This research aims to (1) extract and analyze secondary metabolites in essential oils (EO) of Citrus sinensis peel (a), Citrus nobilis peel (b), Citrus reticulata L. peel (c), and Citrus maxima [Burm]. peel (d) using GCMS, (2) separate and analyze secondary metabolites in the fractions of each EO, (3) observe the anti-inflammatory of each EO by bovine albumin denaturation method, (4) analyze all secondary metabolites by in silico anti-inflammatory and drug-likeness. In this current project, the EO(a) and EO(b) were extracted using hydrodistillation, while the EO(c) and EO(d) were extracted via the hydro-steam distillation method. The research results show that EO(a), EO(b), EO(c), and EO(d) were 0.42%, 0.57%, 0.50% and 0.35%, respectively. Each EO that has been obtained was fractionated at four boiling point levels 80-90 °C, 90-95 °C, 150-160 °C and above 160 °C. The GCMS analysis showed that (d/l)-limonene was the main compound in EO(a) 44.10 %, EO(b) 42.25 %, EO(c) 31.75 %, and EO(d) 46.03%. The fraction of (c-1) contains the highest limonene (96.26%). Anti-inflammatory tests revealed IC50 of 0.5545 mg/ml (a), 1.0049 mg/ml (b), 0.7509 mg/ml (c), and 2.8229 mg/ml (d). In silico analysis shows trans-β-caryophyllene and valencene have the optimum complex binding energy with the 6QS9 and 2BXE. Drug-likeness observations show that all secondary metabolites are safe orally consumed. This finding complements scientific information on citrus peel as an anti-inflammatory and preliminary research for drug development from bioactive natural resources.

Abstract : The aim of the present study was to compare the microleakage of a self-adhesive composite (SAS) and a conventional resin-based fissure sealant using different application techniques. 100 intact human premolars with well-delineated pits and fissures were used and divided into 5 groups (n = 20). Group 1 specimens were etched (37% phosphoric acid) and sealed with conventional resin-based sealant (Helioseal F, Ivoclar Vivadent). In Group 2 Helioseal F was applied with bonding agent. For Group 3, pits and fissures were sealed with (Constic, DMG, Hamburg, Germany) according to the manufacturer’s instructions. In Groups 4 and 5, specimens were sealed with Constic after enamel etching, but Group 5 bonding agent was also applied. Subsequently, specimens were thermocycled (1800 cycles, dwelling time of 10 s), immersed in 2% Methylene blue solution (24 h). Marginal leakage (dye penetration depth) was evaluated under a stereomicroscope and the worst score of each specimen was recorded (I⁻IV). Helioseal F showed the lowest microleakage (Helioseal F: 77.5% scored 0), regardless of bonding agent application (p = 0.200). Microleakage in groups sealed with Constic (with and without bonding agent) were not different (p = 0.449). The quality of marginal sealing after etching was improved when Constic was used (p = 0.000). The present findings suggest that the conventional resin-based sealant provides better marginal sealing than SAS. Additional enamel pretreatment with 37% phosphoric acid reduces marginal microleakage of SAS. Bonding agent application do not enhance the marginal sealing of resin-based sealant.

Abstract : Online motorcycle taxi drivers are frequently exposed to oxidative stress originating from air pollutants. Vitamin 25(OH)D is one of the potent antioxidants in the body. Studies on the relationship between vitamin 25(OH)D concentration and oxidative stress markers in online motorcycle taxi drivers are still rare. Objective of this study was to determine the differences between oxidative stress biomarkers levels in online motorcycle taxi drivers and control subjects, and the relationship of 25(OH)D concentration and oxidative stress biomarkers in both groups. This cross-sectional study involving 70 online motorcycle taxi drivers and 70 subjects as a control, aged 20-50 years. The study was conducted from September 2022 to March 2023. Subjects meeting inclusion criteria were asked to fill in a questionnaire, then underwent examination of vital signs and anthropometric data, after which 10 mL of their venous blood was drawn for examination of 25(OH)D, 8-OHdG, and MDA. Spearman’s correlation and T-test was used with p <0.05 indicating significant difference. Mean age of online drivers was 36.4±7.9 years, whereas mean age of control subjects was 38.5±6.7 years, There was significant differences between 8-OHdG and MDA concentration at online drivers and control subjects. There was no significant correlation of 25(OH)D concentration with MDA and 8-OHdG concentrations in both groups.

Abstract : This study aims to compare the compressive between bulk composite and fiber reinforced bulk composite. This study included 30 cylindrical samples, were randomly divided into two groups in the following order: Group 1 (n=15): Bulk composite (Tetric® N-Ceram Bulk Fill, Ivoclar Vivadent, USA), and Group 2 (n=15): Fiber reinforced bulk composite (Polydentia, Switzerland). These samples were restored by means of a dark Teflon mold for the research, which contains in its center a cylindrical cavity of dimensions: 5 mm in diameter and 6 mm in height. The samples were photopolymerized using light-curing unit LED (Light Emitting Diode), an optical intensity of 1200 mw/cm2, and were stores at 37° for 24 hours before testing, then were subjected to thermal cycles 5000 times between 5° and 55° in a 15 second dwell period, with a 15 second transfer. The samples were prepared for testing and then transferred to the standard testing machine (Testometric AX 114, Universal Testing machine). Results were statistically analyzed using Student's t-test. It was showed that there were statistically significant differences between the two different study groups (p<0.05). The values of microhardness (HV) in the fiber reinforced bulk composite group were higher than in the bulk composite group.

Abstract : The purpose of this study was to use systematic review techniques to look at the relationship between IBS and OCD. We conducted a thorough search of PubMed, SCOPUS, Web of Science, and Science Direct to find pertinent literature. Rayyan QRCI was utilized during the entire process. We included six studies with a total of 970 patients, and 351 (36.2%) were males. Five studies investigated the prevalence of OCD in 340 IBS patients and was found to be 107 (31.5%). Only one study a 10 (47.6%) of IBS in 21 OCD patients. There is a substantial correlation between OCD prevalence and factors like males, younger age groups, single people, and OCD history in close family members of IBS patients. Certain psychological traits and neuroendocrine markers, particularly plasma cortisol and neuropeptide Y, were found to be significantly correlated in IBS patients; in diarrhea-predominant IBS, a correlation between plasma cortisol and serotonin was discovered. IBS patients have a high OCD prevalence, and there is a substantial correlation between OCD and the study's IBS criteria. Consequently, to treat this patient group, gastroenterologists, psychiatrists, and psychologists sought assistance; treatments ought to be carried out collaboratively. To validate the current findings, it is also advised that additional prospective studies look into the relationship between IBS and other psychiatric disorders.