Search Results

Abstract : This study aims to investigate the effect of Lumbricus rubellus extract on serum FOXP3, Interleukin-5 (IL-5) levels, and Bacterial Inhibitory in Atopic Dermatitis patients. A true experimental study with pre-test and post-test procedures and a control group design was conducted. A combination of Lumbricus rubellus capsule extract was given orally to the treatment group and examined by ELISA to determine levels of FOXP3 and Interleukin-5 on day 0, day 14, and day 30. The control and intervention group applied by Vaseline topically. Also, a Bacterial Inhibitory Test was performed in the Clinical Microbiology Laboratory. Of 30 participants, the participants predominated females (53,33%) and those aged 17-25 (23,33%). Lumbricus rubellus Extract can increase FOXP3 levels (3.74 ± 1.44 vs. 3.25 ± 0.86 vs. 2.00 ± 0.99 in week IV, week II, and week 0, respectively; p=0.000) and reduce IL-5 (251.9 ± 248.53 vs. 266.57 ± 271.73 vs. 289.28 ± 255.28 in week IV, week II, and week 0, respectively; p=0.000). A comparable result was found in the control group. In vitro tests revealed no significant inhibitory concentration of the extract. Alternative therapy with Lumbricus rubellus extract can be used in Atopic Dermatitis patients.

Abstract : Protein hydrolyzate is the result of enzymatic or chemical hydrolysis of proteins containing lower molecular weight peptides and free amino acids. Making protein hydrolyzate is an effort to increase protein sources rich in amino acids. The general objective of this study was to utilize Pokea shells (Batissa violaceae celebencis Marten, 1897) from Pohara Village, Konawe, Southeast Sulawesi Province, as an enzymatic protein hydrolyzate product that can be provided in nutraceutical form. This study consisted of two stages, the first stage was the determination of enzyme concentration and optimum hydrolysis time. Then the second stage was the production of protein hydrolyzates under optimum conditions and screening of the resulting protein hydrolyzate products. Hydrolysis of proteins was carried out with the enzyme papain. Screening was done to identify groups of chemical compounds in protein hydrolyzate products. The results of the first stage of this study showed that the concentration of papain enzyme 6% (w/v) of the total substrate volume and hydrolysis time of 48 hours gave the best result, which was a yield of 50%. The higher yield of protein hydrolyzate products indicates that more protein is hydrolyzed. Based on the results of proximate analysis conducted on protein hydrolyzate products in the form of powder, water content of 9.77%, protein content of 79.58%, fat content of 2.95%, ash content of 8.52%, and carbohydrate content of 0.1 0% were obtained. Based on the results of the chemical compound group content test, protein hydrolyzate products from Pokea shells contain alkaloid compounds.

Abstract : The choice of the retrograde filling material with desirable qualities is essential for the success of periapical surgical therapy, including biological acceptability, good sealing ability, non-toxicity, periapical tissue healing, ease of application, dimensional stability, and resistance to moisture. The purpose of this study was to assess the effectiveness of apical sealing using BC-RRM (sealer + putty), and Biodentine after apicectomy. Thirty single-rooted extracted human teeth with single canals were collected. Each tooth had no morphological defects and major carious lesions. All teeth were sectioned at the Cementum- Enamel junction. Root canals were instrumented with rotary files until #25 size, 4% taper, and were obturated using a single–cone technique with Zinc Oxide Eugenol sealer, and then the samples were stored in an incubator in 100% humidity at 370C for 24 h, and then 3 mm of the apical third were resected at 900 angle according to the long axis of the root. The sample was randomly divided into three groups (n=10) (BC-RRM, Biodentine, control group) The teeth were stored in an incubator in 100% humidity at 370C for 24 h, then the root surface was isolated with two layers of nail varnish, except for 3 mm at the apex. The samples were immersed in 2% methylene blue dye for 36 hours, and then the root was sectioned longitudinally to evaluate the depth of dye penetration between the canal wall and the retro-filling materials under optical magnification. The data were statistically analyzed using (Mann-Whitney U) (α= 0.05) (Kruskal-Wallis). There was no statistically significant difference in the apical sealing between the Biodentine group and BC-RRM group. When employed in the retrograde filling instead of the MTA material, the BC-RRM materials and the Biodentine demonstrated advantages in apical sealing.

Abstract : The positive effect of blood concentrates on the healing of soft and hard tissues in periodontal and peri-implant surgeries has been proven. The growth factors contained in blood concentrates are the source of these positive properties. Injectable platelet-rich fibrin (I-PRF) is one of the recent generations of these extracts and its clinical form helps to be added to soft tissue grafts. I-PRF releases growth factors during the healing of these grafts. Therefore, our study aimed to evaluate the healing of the recipient site following the application the connective tissue graft to augment the peri-implant soft tissues. The research sample included 20 sites that needed peri-implant soft tissue management. In the test group, I-PRF was added to the Tuberosity connective tissue graft before it was sutured at the recipient site, while in the control group, the Tuberosity connective tissue graft was used at the recipient site without additions. Wound healing was assessed at weeks 1, 2, 3, and 4 to evaluate the ability of I-PRF to enhance the healing. Statistical tests were performed using SPSS v.22. The results showed that the soft tissue healing was similar in both groups with a minimum preference for the test group. The values of the significance level were greater than 0.05 when comparing the two groups in all observation periods, which means that the differences were not statistically significant. This study found that I-PRF enhances the healing of soft tissue wounds around implants when added to the connective tissue grafts. However, this result still needs more studies to be proven.

Abstract : Stunting is a nutritional problem that can be dangerous for babies because it can cause emotional, social, and cognitive development problems in adulthood. Multidimensional factors cause stunting, so prevention does not only focus on nutritional status. This study aims to test the early warning model for increasing family knowledge, support, and attitudes to prevent stunting. The method was a pilot study with one pretest and a posttest treatment group. The sample for this study was 90 families who had pregnant women and met the criteria, where the sampling technique used purposive. The trial program was carried out for two months. Data analysis using the Wilcoxon Signed Ranks Test and Paired sample T-test. The results showed that the increase in post-test knowledge was an average of 21.62, an increase in family support at the post-test was an average of 16.5, and an increase in family attitudes during the post-test was an average of 19.28. Bivariate analysis showed that there was an increase in average post-test knowledge (p=0.000), there was an increase in family support (p=0.000) and an increase in family attitudes in the post-test (p=0.000). This result proves that there is a significant increase in the three variables. This model can also encourage improved family attitudes in a better direction, so it is expected to advance changes in people's behaviour in preventing stunting.