Abstract : The choice of the retrograde filling material with desirable qualities is essential for the success of periapical surgical therapy, including biological acceptability, good sealing ability, non-toxicity, periapical tissue healing, ease of application, dimensional stability, and resistance to moisture. The purpose of this study was to assess the effectiveness of apical sealing using BC-RRM (sealer + putty), and Biodentine after apicectomy. Thirty single-rooted extracted human teeth with single canals were collected. Each tooth had no morphological defects and major carious lesions. All teeth were sectioned at the Cementum- Enamel junction. Root canals were instrumented with rotary files until #25 size, 4% taper, and were obturated using a single–cone technique with Zinc Oxide Eugenol sealer, and then the samples were stored in an incubator in 100% humidity at 370C for 24 h, and then 3 mm of the apical third were resected at 900 angle according to the long axis of the root. The sample was randomly divided into three groups (n=10) (BC-RRM, Biodentine, control group) The teeth were stored in an incubator in 100% humidity at 370C for 24 h, then the root surface was isolated with two layers of nail varnish, except for 3 mm at the apex. The samples were immersed in 2% methylene blue dye for 36 hours, and then the root was sectioned longitudinally to evaluate the depth of dye penetration between the canal wall and the retro-filling materials under optical magnification. The data were statistically analyzed using (Mann-Whitney U) (α= 0.05) (Kruskal-Wallis). There was no statistically significant difference in the apical sealing between the Biodentine group and BC-RRM group. When employed in the retrograde filling instead of the MTA material, the BC-RRM materials and the Biodentine demonstrated advantages in apical sealing.