Search Results

A comparative study of the effectiveness of Pfizer-BioNTech (BNT162b2), AstraZeneca (ChAdOx1nCoV-19) and Sinopharm (BBIBP-CorV) vaccines in eliciting Cell mediated immunity in a sample of vaccinated population from Iraq.
Journal ID : AMJ-03-09-2022-10507; Author : Furqan mohammed, Ahmed sahib,
Abstract : In order to tackle COVID-19 pandemic and the emerging variants, researchers around the globe have investigated many vaccine candidates from different manufacturers, however vaccine development is not an easy task but is a top priority to restore normalcy as represented a step to achieve the desired herd immunity threshold. In this study we assessed and compared the level the peripheral mononuclear cells proliferation (PBMC) activity and in vitro INF-gamma release by the S1 spike protein stimulated T cells as they represent cellular immunity triggered from each vaccine against SARS-CoV2 infection in 123 vaccinated supposedly not infected subjects, by using Microculture tetrazolium assay (MTT) proliferation assay and an ELISA technique for the assessment of INF-gamma level in a cell culture supernatant. This study findings indicated that there was significant variety in the elicited levels of cell mediated immunity by dual vaccination at 1month and much more sustained different levels of cellular immunity among the studied previously mentioned vaccines by month8. Interestingly, both the PBMC proliferation percentage and the concentration of the in vitro IFN-y release, which represent the cellular immunity to SARS-CoV-2, were remarkably higher in 8 months than in 1month post-2nd dose vaccination groups of the three vaccines studied in this study (P<0.0001). For the PBMC proliferation percentage, AstraZeneca vaccine induced much higher proliferation percentage than Pfizer and Sinopharm (P<0.01) which both showed very close PBMC proliferation (P>0.05). For the concentration of the in vitro lFN-y release, again AstraZeneca induced distinguishing higher levels than Sinopharm and Pfizer groups (P<0.0001); nevertheless, unlike the trend found with the PBMC proliferation, Pfizer group showed significantly higher concentration of the in vitro lFN-y release than Sinopharm group (P<0.05). Moreover, the cellular immune response to all three vaccines studied showed no relationship with age, sex, and comorbidities of the vaccinated population of the study. Taken together, AstrZeneca showed superior effect on inducing robust cellular immunity followed by Pfizer vaccine, while Sinopharm showed minimal cellular immune response induction. And for all vaccines studied, the cellular immunity increased with time over 8 months after vsccination.
Read more →

Association between stress and caries experience in primary schoolchildren
Journal ID : AMJ-02-09-2022-10506; Author : Assis. Lecturer. Shahad Jamal,
Abstract : Stress can make you more susceptible to tooth caries through four different processes. According to research, adolescents are more likely to acquire poor body image, disordered eating habits, and high levels of Stress. Students experience stress due to competition and the massive quantity of material they must learn in a short period of time; studying for and taking examinations are the most frequent causes of academic stress. The goals were to examine caries experience in connection to perceived stress in children aged 6 to 12 years. A cross-sectional study will carry out involving a sample from the Pediatric Dentistry department of Al-Farahidi University, Faculty of Dentistry. The questionnaire included demographic information as well as the Perceived Stress Scale (PSS). Using the WHO's (1997) diagnostic standards for caries for missing, decayed, and filled teeth, dental caries status was examined (DMFT). Dental caries was 49.5% prevalent. The average DMFT was 14.3%. Weak (r=o.37o) (p Read more →

Effect of surface treatment on bond strength of new fiber post system (Rebilda GT) using different luting agents.
Journal ID : AMJ-02-09-2022-10505; Author : Emad F. Alkhalidi, Alaa E. Dawood,
Abstract : The aim of the present study was to evaluate the push-out bond strength of Rebilda bundle fiber posts cemented using different luting cements. Roots of thirty single-rooted mandibular first premolars were used in the present study. The root canals were instrumented using Protaper files and obturated with size X3 guttpercha. A space for the post was created by removing the gutta-percha from the coronal and middle thirds of each root using peeso reamers leaving 5mm apically to maintain apical seal. The roots were randomly divided into three groups (10 each). Ten roots were filled by Rebilda bundle fiber posts cemented according to the manufacturer instructions using Rebilda DC kit. Ten roots were filled by Rebilda bundle fiber posts cemented with self-adhesive resin cement. Ten roots were filled by Rebilda bundle fiber posts cemented with resin-modified glass-ionomer cement (GIC). Each root was cut into three 2mm-thick slices (cervical, middle and apical) and 30 specimens were prepared for each group. The slices were marked and labelled to ensure that the load is applied apico-cervically. Universal testing machine was used to measure the push-out bond strength. The push-out bond strength of the posts at the cervical root slices was significantly higher than the push-out bond strength of the posts at the middle and apical root slices. For the cervical and middle root slices, there were no significant differences between bond strength values of the posts cemented using Rebilda DC kits and the posts cemented using self-adhesive resin cement. The use of resin-modified GIC for posts cementation produced the lowest push-out bond in comparison with the other luting agents. It might be possible to recommend the cementation of bundle fiber posts using self-adhesive resin cements (with-out adhesive bonding agent) or resin-modified GIC.
Read more →

Evaluation of The Antibacterial Activity of the Alcoholic Extract of Nasturtium officinale (watercress) with Calcium Hydroxide against Enterococcus faecalis Isolated from Root Canal
Journal ID : AMJ-02-09-2022-10504; Author : Mustafa W. Abdul Kareem, Zainab Abdul jabbar Al-Dhaher,
Abstract : Herbal extracts exhibited a worth noticed antimicrobial activity against wide range of microorganisms. Apparent worldwide interest in the production of high value phytochemicals, this new approach of using natural remedies termed phytotherapy or ethnopharmacology. Root canal treatment protocols require the application of effective intracanal medicaments especially in cases with infected or previously treated canals in order to eradicate resistant microorganisms and maximize the success rate of the treatment. Nasturtium officinale is a plant with documented beneficial properties. Many studies investigated its antimicrobial properties and the results were positive. The aim of the study was to investigate the antibacterial activity of the alcoholic extract of Nasturtium officinale (watercress) alone and in combination with calcium hydroxide against Enterococcus faecalis as intracanal medication. Enterococcus faecalis is widely recovered from infected canals and regarded the most commonly isolated bacterial species from failed treated cases. The sensitivity of this bacteria to different concentrations of alcoholic extract of Nasturtium officinale was determined by using agar well diffusion method in comparison to calcium hydroxide paste. The minimum bactericidal concentration (MBC) of the tested extract against the isolated bacteria was obtained by agar plate method. Another experiment involving testing the combination of the extract with calcium hydroxide was evaluated and compared to calcium hydroxide paste with iodoform. The results of the study were promising and the extract exhibited an antibacterial activity against Enterococcus faecalis by formation of growth inhibition zones. The highest concentration tested (20 mg/ml) demonstrated the largest diameter of inhibition zones in Muller Hinton Agar plates. The studied combination with calcium hydroxide revealed a positive reaction. In conclusion, alcoholic extract of Nasturtium officinale proved to have effective antibacterial activity against Enterococcus faecalis and the combination led to enhancement of the antibacterial activity of calcium hydroxide.
Read more →

Evaluation of horizontal alveolar bone augmentation with mandibular bone block harvested from retromolar region using the split bone block technique
Journal ID : AMJ-02-09-2022-10503; Author : Mustafa Jasim Mohammed, Consultant Dr. Waleed Khalil Ismail,
Abstract : Reconstruction of alveolar defects after tooth loss is one of the biggest challenges in implant dentistry, to augment bone defects grafts of various origins have been used, the mode of graft integration depends primarily on its origin and composition. Among graft material used is the autogenous bone, which considered the gold standard for augmentation due its osteoinductive, osteogenic, osteoconductive with significant regenerative capacity over all other grafts. Intraoral sources of autogenous bone are considered the preferred donor site, due to proximity of donor and recipient sites, convenient surgical access, low morbidity, and elimination of a hospital stay. Severe alveolar defects necessitate using sufficient quantity of structurally resistant bone block for reconstructions, cortico-cancellous bone block grafts suitable for two- or three- dimensional reconstruction of these defects can be harvested only from mandibular symphysis and retromolar area. Radiographic evaluation of the outcomes of horizontal defect reconstruction with autogenous bone block harvested from retromolar area in terms of amount of horizontal bone gain and graft density four months postoperatively. This study included 10 patients for reconstruction of horizontal ridge defect using autogenous bone block harvested from retromolar region, the patients were treated at the oral and maxillofacial surgery unit of Al-Yarmouk teaching hospital and private clinic during the period from January 2020 to October 2021, the age of patients ranged from 20 to 53 years old, all patients were medically fit to undergo a bone grafting procedure. Cone Beam Computed Tomography was used for assessment of bone density and horizontal bone width pre-operatively and 10 days, 4 months post-operatively. Post-operative complications were assessed and recorded during the follow-up period. In this study, the females’ percentage was higher than males (70% versus 30%). The mean of the pre-operative width of alveolar defect was (2.54 ± 0.42) mm, after 4 months the mean of bone width gain was (4.480 ± 0.823) mm. The density of the pre-augmentation site measured with a mean of (784.8 ± 183.8) HU, then after 4 months of bone grafting procedure the density of grafted area also measured, the mean was (666.8 ± 106.4) HU. All patients developed swelling post-operatively and resolved gradually, 90% of patients complained of pain during the first week postoperatively, while only (10%) suffered from sensory disturbance for 2 weeks after the operation. Mandibular ramal bone block used according to the split block technique is a predictable method for augmentation of sever horizontal defects, autogenous bone grafts remain the gold standard of grafting materials, Cone Beam Computed Tomography is recommended, as these scans provide precise information about the thickness of bone and position of inferior alveolar nerve, the use of piezo surgery unit led to precise and clean harvest graft with minimal wastage of bone, and the overall morbidity of mandibular block for ridge augmentation is minimal.
Read more →