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Anti-proliferative activity of Cassia angustifolia in Breast Cancer Cell Line
Journal ID : AMJ-06-02-2022-10164; Author : Walaa Najm Abood,
Abstract : Medicinal plant was wildly used at recent year for treated many diseases instead of chemical drugs because of less in side effect. Cassia angustifolia leaves extract was investigated in this study as anti-proliferation agent for type of breast cancer cell. C.angustifolia (CA) ethanol leaves extract and its fractions (CAF) were evaluated in vitro for the MCF7 cell line and MDA-MB-231 cell line as anti-proliferation. The cells were treated in different doses for CA and CAF at 24h and 28h. The treated cells were stained with AO/EB stain to evaluated apoptosis effect of CAF. GCMS method was used to identify active component in the CAF that give highest anti-proliferation activity. Leaves extract of C.angustifolia was identified as anti-proliferation for MCF7 cell line and MDA-MB-231 cell line. The result was revealed that CAF1 was had highest inhibition percentage for both cells, in (76.73 ± 0.23) % for MCF7 cell and (70.49 ±2.6) % for MDA-MB-231cell. Apoptosis percent present for treated MCF7 cell at 24h and 48h was (84.32 ±22.42) and (72.32 ± 25.08) respectively. And for treated MDA-MB-231cell was (60.16 ± 40.13), (65.31 ± 30.28) respectively for the highest dose 1000 µg/mL. GCMS analysis was identified 23 active component as 2-Pentanone, 4-hydroxy-4-methyl- and 2H-Pyran, 2-(2-heptadecynyloxy), Hexadecanoic acid, methyl ester, Oxalic acid, Phthalic acid, di(2-propylpenty, 2-Pentadecanone, 6,10,14-trimet,and Carbonic acid, decyl tridecyl ester, 2-Tetradecynal, 4-hydroxy-, Tritriacontane, Tricosane, Hexadecanoic acid, methyl ester and Hexadecanoic acid, ethyl ester, Pentadecanoic acid, 14-methyl ester, Heptadecanoic acid, 16-methyl- , Methyl 8-oxooctanoate, Hexadecyl propyl ether, Undecanal, Octadecanoic acid, ethyl ester, 3-Methyl-4-(phenylthio)-2-prop-, dimer of Coleon F. In conclusion that C.angustifolia frication1 for ethanol leaves extract has anti proliferation and antitumor effect on the breast cancer by enhanced apoptosis pathway that lead to programmed cell death for cancer cell and prevent metastasis of its. For that CAF1 could be as new drug to treat aggressive breast cancer.
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Wound Remodelling Anal Trauma in Wistar Rat After Application of Combination Platelet Rich Plasma (PRP) and Stromal Vascular Fraction Cells (SVFs)
Journal ID : AMJ-09-01-2022-10159; Author : Ades Santa Satria, Sulmiati, Nita Mariana, Andi Alfian Zainuddin, Fonny Josh, Sarachaswaty L. Laiding,
Abstract : Anal trauma is a very rare condition. Although rare, anal trauma has the potential to cause anal stenosis, ectropion, or incontinence that can interfere with a person's quality of life. This problem can also be caused after surgery on the anus, which is the primary modality of this case. More severe complications can include peritonitis, sepsis, to death. Therefore, an effective non-invasive alternative therapy is urgently needed in healing anal trauma. This research was conducted using an experimental laboratory method on male Wistar rats using a post-test control group consisting of three groups treated with PRP+βSVF (each, n=4), three groups treated without PRP+SVF (each, n=4), and one control group (sacrifice on day 0). All the treatment groups were sacrificed on days 1, 7, and 14 post-treatment. The anal trauma was made through a longitudinal incision from distal to proximal with a depth of 5 mm from serosa to intraluminal. TGF-β was measured using the ELISA method. Data represented the mean +/- standard deviation and was further analyzed by the one-way Anova test. There was a significant difference between the mean TGF- β levels in the intervention group and the control group on day 1 (31.07±1.65 vs. 25.6±1.02, p=0.01) and day 14 (31.47±0.51 vs. 27.42±1.17, p= 0.01). Meanwhile, on day 7, there was no significant difference (29.37±2.27 vs. 26.44±2.61, p=0.18). A combination of SVF and PRP effectively accelerates the healing process of anal trauma in vivo, which is assessed based on TGF-β levels.
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DIARRHEAL INFECTIONS IN UKRAINE: CURRENT EPIDEMIOLOGICAL SITUATION
Journal ID : AMJ-02-12-2021-10158; Author : Malysh Nina, Chemych Mycola, Doan Svitlana, Rodyna Roman, Chorna Inna,
Abstract : Purpose of the study on the background of the analytical study of the incidence of diarrheal infections in Ukraine under the current conditions, to determine the factors influencing epidemic situation. The processes of globalization, fundamental changes in nutrition and water consumption contributed to the fact that the significance of individual infection sources, ways of invader transmission, etiological structure of diarrheal infections changed. Epidemiological and statistical research methods are applied. Statistical processing of the study outcome is carried out using software application Microsoft Office Excel 2010, Statstica 6. The epidemic situation with diarrheal infections in Ukraine is characterized by a low incidence of typhoid fever, shigellosis, stable incidence rates without the downward trend, salmonellosis, high incidence of diahrreal infections of specified etiology and diahrreal infections of unspecified etiology. The most complicated epidemic situation is observed in economically most developed regions of the country. Most diarrheal infections outbreaks are connected with catering establishments 36.5 % and with children educational establishments 26.1 %. In the region with the highest shigellosis and salmonellosis incidence direct strong correlation relationships are established between the incidence and population, density, natural population movement. The oblasts with the highest diahrreal infections of specified etiology, rotaviral enteritis, diahrreal infections of unspecified etiology incidence are at least provided with water resources and have problems with provision of high-quality drinking water.
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Modern approaches to assessment toxicity of xenobiotics
Journal ID : AMJ-18-09-2021-10153; Author : Garaev, Huseyngulieva,
Abstract :

Consumers are exposed to a diversity of chemicals in all areas of life. Air, water, soil and food are all unavoidable components of the human environment. Each of those elements influences the quality of human life, and each of them may be contaminated. We are exposed to toxic or potentially toxic compounds in many ways in our daily lives and toxicology is clearly a subject of great importance for society. This becomes apparent when we look at the types of poisons and the ways in which we are exposed to them. Indeed, the categories cover virtually all the chemicals one might expect to encounter in the environment. After consideration of this, one might well ask “Are all chemicals toxic?” Phrase as an answer: “There are no safe chemicals, only their safe use”. Xenobiotics are defined here as those compounds, both organic and inorganic, produced by human beings and introduced into the environment, as well as into the food chain at concentrations that cause undesirable effects. Xenobiotics in the food chain are monitored in two forms: by testing – the objective of which is to discover unsuitable foodstuffs in the consumer’s network, and by monitoring – to obtain objective information about environmental components contamination and to harmlessness health of available foodstuffs.

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Characteristics of anxiety and depressive manifestations in patients with acute myocardial infarction taking into account their personal accentuations
Journal ID : AMJ-18-09-2021-10151; Author : Boiko, Kachur, Ajala, Bodnar, Zhyvotovska,
Abstract :

The main objective of the study was to find out prevalence of depression and anxiety symptoms in the population of patients with AMI with ST-segment elevation (STEMI), treated with primary PCI (pPCI). Secondary target indicators included the incidence of sleep disorders and loss of interest in sex. The project enrolled 79 consecutive patients with the first AMI, aged <80 years (median 61 years, 21.5% of women) with a follow-up period of 12 months. Symptoms of depression or anxiety were measured using the Beck Depression Inventory II tests (BDI-II, cut-off value ≥14) and Self-Rating Anxiety Scale (SAS, cut-off ≥ 45) within 24 hours of pPCI, before the discharge, and in 3, 6 and 12 months). Results with the value p<0.05 were considered as statistically significant. The BDI-II positivity was highest within 24 hours after pPCI (21.5%) with a significant decline prior to the discharge (9.2%), but with a gradual increase in 3, 6 and 12 months (10.4%; 15.4%; 13.8% respectively). The incidence of anxiety showed a relatively similar trend: 8.9% after pPCI, and 4.5%, 10.8% and 6.2% in further follow-up. Patients with STEMI treated by primary PCI have relatively low overall prevalence of symptoms of depression and anxiety. A significant decrease in mental stress was observed before discharge from the hospital, but in a period of one year after pPCI, prevalence of both symptoms was gradually increasing, which should be given medical attention.

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