Abstract : Highly contagious hepatitis B virus (HBV) might result in both chronic and acute hepatitis. The purpose of this work was to look into the level of anamnestic and vaccination response to a booster dosage of recombinant-Hepatitis B vaccine among healthcare professionals. In a cross-sectional study involving 232 healthcare professionals, sera were tested using an enzyme-linked immunosorbent assay for the presence of hepatitis B surface antibodies. The anti-HBs antibody titer was measured before and following a booster dose of recombinant hepatitis B vaccination to evaluate the anamnestic response. The study had shown that protective level regarding anti-HBs antibody (≥10mIU/ml) was observed in 170(73.3%) HCWs while 62(26.7%) of them had titer of (<10 mIU/ml). Statistically significant differences were demonstrated among different profession groups with highest Anti-HBs titer ≥100 IU/mL observed among the vaccination staff 8(100%). On contrary highest percentage of ˂10mIU/mL anti-HBs titer was observed among cleaners 28(47.5%). A significant correlation was demonstrated between the protective titer and the number of vaccine doses received and the time elapsed after the last dose of vaccine. Forty-one participants 41/62(66.1%) with AntiHBs titer<10 mIU/ml agreed to receive a booster dose of vaccination, and six weeks later, all gained titer >10mIU/ml. Vaccination coverage rate among HCWs is still low; those who did not respond well to the primary vaccination course need to receive booster doses. The infection control and prevention department (ICP) in hospitals and health facilities must concentrate its efforts on raising awareness among all HCWs.