Abstract : Vitamin D can be obtained through foods of plant and animal origin, supplements, or synthesized endogenously through skin exposure to sunlight. Despite adequate sun exposure, vitamin D deficiency may still occur. In the bloodstream, vitamin D is transported by albumin; thus, hypoalbuminemia can reduce circulating vitamin D levels. To determine the impact of low vitamin D dietary intake and limited sun exposure on vitamin D levels after 30 days. This study involved 32 female rats divided into control (K) and treatment (P) groups. Rats were fed a pellet diet low in vitamin D, derived from fish meal and corn. They were exposed to sunlight for only 90 minutes daily (10:00–11:30 PM) over a 30-day period in a light-proof room. ELISA tests were performed in the third and fourth weeks. One-way ANOVA showed significant differences in vitamin D levels between K3–P4 (p=0.049), P3–P4 (p=0.003), and K4–P4 (p=0.047). Albumin levels also differed significantly between K3–K4, K3–P4, and P3–P4 (p=0.000). The study demonstrated that reduced dietary intake and limited sunlight exposure significantly affected both vitamin D and albumin levels. There is a correlation between vitamin D deficiency and decreased albumin levels. Reduced albumin synthesis may impair the metabolism and absorption of vitamin D, serving as a potential marker of malnutrition and inflammation severity.