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Abstract : About 200 samples of various patients who were hospitalized to the general hospital, the teaching hospital for women and children, and the Al-Diwaniyah teaching hospital between November 2020 and May 2021 yielded a total of 20 Shigella species isolates. The isolates were identified using traditional methods and polymerase chain reaction technique, named as No.1 to No.20. The whole genomic DNA of twenty isolates, as No.1 to No.20 isolated from different geographic regions of Iraq, was extracted. The sequencing of 16S ribosomal RNA gene pathogenic Shigella spp. was performed, and the sites of these genes on the genome of the isolates were determinate. The phylogenetic tree analysis, using MEGA X10.2.4 software program was achieved, and the matching of the current study isolates with NCBI-Gen bank global Shigella strains, elicited that the phylogenetic tree genetic relationship analysis showed that the local Shigella spp. (IQ-No.1 to IQ-No.20) isolates were showed closed genetics related to NCBI BLAST Shigella spp. strain SK8 Indian isolates (MW600518.1) at total genetic change (0.0040-0.0010%).