A total of 150 people (patients (118) and controls (32)), were recruited for the study. During the period from September 2021 to February 2022, patients diagnosed by Ophthalmic doctors to have bacterial eye infections (blepharitis, conjunctivitis, or keratitis) were out patients of (Ghazy Al-hariri Hospital and Ibn Al-Haytham Hospital in Baghdad), while controls were collected from different locations. They were of both sexes and ranged in age from 20 to 60 years old. Blood and eye swabs were collected from all participants. A total of (72) gave a P+ve cultures while (78) gave a N-ve cultures, identify by VITEK2 system. Both sexes suffer from eye infections, with females representing the majority (62%) and males representing (38%).The most prevalent pathogenic bacteria were Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa ,Klebsiella pneumoniae, Pasteurella pneumotropica, Corynebacterium spp, while Leclerica adecarrboxylata, Proteus mrabilis, Rhizobium radiobacter, Pseudomonas luteola, Staphylococcus lentus, Staphylococcus sciuri, Staphylococcus simulans and Kocuria rosea were the lest with one isolate of each. The results also show that vitamin D3 has a big effect on the immunity of the people who took part in the study. In other words, people who have a deficiency in vitamin D3 are more likely to get an eye infection.