Abstract : Sepsis is a life-threatening organ dysfunction caused by a dysregulated host response to infection. Andrographis paniculata (AGP) was anti-inflammatory by inhibiting nuclear factor-kappa B (NF-κB), a family of transcription factors that plays a central role in the inducible nitric oxide synthase (iNOS) expression. To prove the effect of AGP on NF-κB p65 and iNOS expression in lipopolysaccharide-induced liver injury in a rat model of sepsis. Immunohistochemistry (IHC) was performed on 30 liver paraffin blocks from 5 groups of male Wistar rats: K1: healthy control; K2: negative control; P1, P2, and P: rats had given AGP doses of 200, 400, and 500 mg/kg BW/day for 14 days. The mean NF-κB p65 and iNOS expression scores in the K1: 3.83±0.408 and 2.83±0.753; K2: 4.00±0.000 and 3.67±0.516; P1: 3.50±0.837 and 3.50±0.837; P2: 3.83±0.408 and 3.83±0.408; P3: 3.83±0.408 and 3.67±0.516. AGP doses of 200, 400, and 500 mg/kg BW/day did not affect NF-κB p65 and iNOS expression in lipopolysaccharide-induced liver injury in a rat model of sepsis. The NF-κB p65 and iNOS expression had a significant correlation (p<0.05) with a coefficient value of 0.514 (moderate). AGP extract prevents the accumulation of lipids that lead to liver necrosis. NF-κB p65 expression had a significant correlation with iNOS expression (p<0.05).