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Abstract : Dengue fever still occurs in many communities every year, so it needs to be prevented. The research objective was to determine the effect of education on mothers' knowledge about DHF prevention. The sample consisted of 40 mothers who were willing to take part in education in Bandung area. Statistical test using the Wilcoxon Sign Rank Test. The results of the study showed that there were significant differences in mothers' knowledge before and after education. It is necessary to carry out health education about DHF on a regular basis to prevent and improve people's behavior in efforts to prevent DHF.