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Abstract : Fatigue is prevalent and leads to increase risk of nurses’ill health, nurses calling in sick, a high turnover, and increased medical errors. The findings in this study demonstrate the gender difference in nursing fatigue cope strategies to support and inform organisational policy development, education, and communication practice addressing the nursing fatigue coping strategies in Jordan. The study examined how the nurses’ gender in Jordan impacted their fatigue prevalence and adopted coping strategies. A qualitative descriptive design was used to explore nurses’ experience of fatigue through a purposive sample of 27 nurses from across the hospital wards. Qualitative data collection was done using semi-structured interviews administered. The findings were analysed thematically through the grounded theory model, where open coding was used then, followed by an axial coding process to establish relationships between themes and sub-themes. The key themes were (i) nurses’ fatigue coping strategies, (ii) gender and nursing fatigue in Jordan, and (iii) fatigue prevalence among Jordanian nurses. Additionally, the findings suggested two coping strategies (a) nurse individual coping strategies and (b) hospital management based coping strategies. In summary, fatigue is highly prevalent among Jordanian nurses. It can have detrimental effects for both male and female nurses, and inter-shift fatigue recovery differs among both genders. Women were found to experience more fatigue in the workplace. Culture and religious beliefs were perceived to be the key contributing factors to gender fatigue levels variances. Individual strategies such as physical exercise, meditation, and relaxation regimes were found to help in managing nurses' fatigue. Hospital management strategies include working shifts with a maximum of eight working hours, weekly working hours to an average of 40 hours, and increased inter-shift rest periods.