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Abstract : To evaluate the effect of botulinum toxin type A (BTA) injection into five points in the safe zone of masseter muscle on the volume of mandibular angle and masseter muscle width. Thirteen volunteers received a single session of 25 units of BTA into the safe zone of each masseter muscle. Three-dimensional cone bean computed tomography (CBCT) was conducted before injection and after six months of the injection. Masseter muscle thickness and mandibular angle volume were evaluated. Cross sectional areas and masseter muscle volume were significantly reduced after six month of the injection with 9.8 % reduction in masseter muscle thickness. The mandibular angle volume was also significantly reduced. Single session of BTA injection into the safe zone of each masseter muscle is effective in reduction of masseter muscle width and mandibular angle volume.