Abstract : Type 2 diabetes mellitus is a long-term metabdic condition marked by a relative lack of insulin secretion, insulin resistance, or both, it results from an imbalance between oxidants and antioxidants factors. The current research was performed to correlate the concentrations of non-enzymatic antioxidants Glutathione, Ceruloplasmin and the concentration of some trace elements that act as antioxidants Selenium, Zinc, Copper and Iron in blood serum of type 2 diabetes patients compared to healthy control in Basra Governorate _ Iraq by according to gender. In this study a total number of (50) diabetic patients (26 Males, 24 Females) as compared with (43) healthy (11Males, 33 Females). It was found that a highly significant increase (P<0.001) in Ceruloplasmin, Copper and Iron concentrations in all diabeticpatients in comparison to the healthy control. As well as there were a highly significant decrease (P<0.001) in Glutathione, Selenium and Zinc concentration in serum of all diabetic patients as compared to healthy control. Also there were no significant difference between males and females patients in serum concentration Glutathione, Ceruloplasmin, Zinc and Copper, but there was a significantly increase (P<0.05) in serum concentration Selenium and Iron in males than females patients. A weak negative correlation was found between (Zinc and Iron) and it was no significant (P>0.05). Also there were a weak positive correlation between (Copper and Ceruloplasmin), it was no significant (P>0.05). In addition there were a weak positive correlation between (Glutathione and Selenium), it was no significant (P>0.05) in diabetic patients.