Abstract : Furcation perforation presents a significant clinical challenge in endodontics due to its anatomical complexity and the risk of bacterial contamination, periodontal breakdown, and tooth loss. Successful repair depends on selecting materials that offer both effective sealing and mechanical stability under functional stress. This in- vitro study aimed to evaluate and compare the sealing ability and push-out bond strength of three repair materials—Mineral Trioxide Aggregate (MTA-Angelus), Biodentine, and NeoPutty MTA—when used to treat standardized furcation perforations in human mandibular molars. Sixty extracted molars were selected based on strict inclusion criteria and divided into three groups (n=20), each further split into subgroups for sealing ability and bond strength testing. Furcation perforations were created and repaired using the designated materials. Sealing ability was assessed via dye penetration using 2% methylene blue and analyzed with ImageJ software. Push- out bond strength was measured using a universal testing machine, and failure modes were classified under stereomicroscopy. Results showed that Biodentine exhibited the least microleakage and highest bond strength, followed by NeoPutty MTA, while MTA-Angelus demonstrated the highest dye penetration and lowest mechanical retention. The differences were statistically significant (p < 0.001). Biodentine’s superior performance was attributed to its rapid setting, dimensional stability, and bioactivity. NeoPutty MTA showed promising results due to its premixed consistency and hydroxyapatite-forming potential. MTA-Angelus, while biocompatible, was limited by its handling properties and prolonged setting time. In conclusion, Biodentine proved to be the most effective material for furcation perforation repair in terms of sealing and retention, suggesting its clinical advantage in managing such defects.