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Abstract : Bacteria are the biggest cause of infectious diseases in humans. One of these bacteria is derived from Staphylococcus aureus. Staphylococcus aureus can cause a variety of infections from mild to life- threatening. Methicillin Sensitive Staphylococcus aureus (MSSA) is a Staphylococcus aureus bacterium that is still sensitive or not resistant to antibiotics. Colonization of MSSA is one of the causes of infection in Staphylococcus aureus. This study aims to analyze the risk factors for MSSA colonization in productive age in Guji Baru village, West Jakarta. This study is a crosssectional design with 30 subjects aged 20-40 years. Techniques for collecting data and knowing MSSA colonization using questionnaires and research in the laboratory. The data obtained were analyzed using the chi square test. The results showed that the prevalence of MSSA colonization was 33.3% and the risk factor for MSSA colonization was the habit of using towels alternately with other people (p=0.028; OR=1.125-31.989). This shows that the habit of using towels alternately with other people is a risk factor that affects MSSA colonization in productive age residents in Guji Baru village, West Jakarta.