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Abstract : Needlestick injuries still pose a high risk to healthcare workers and a global concern for transmission of 20 blood-borne pathogens such as human immunodeficiency virus and hepatitis B and C viruses. The Centers for Disease Control of the USA estimated that exposure to blood and body fluids with sharp tools and needle stick and sharps injury affects three million health workers annually with an estimated six million national security indicators each year. This study is aimed to estimate the association between the prevalence of needle stick injury among healthcare workers and their demographic data in Baghdad hospitals. A descriptive cross-sectional study design was utilized, (300) health care workers in Baghdad hospitals. The sample selected from three hospitals in Baghdad City who were worked in (Baghdad Teaching Hospital, Al- Yarmook Teaching Hospital and Al- Zafaraniyah General Teaching Hospital), during the period from 25th November 2022 to 20th May 2022. The data was collected through interview technique and use constructed questionnaire format which consists two parts. The data were collected through face to face interview technique and by using questionnaire format. Data were analyzed through the application of descriptive and inferential statistical analysis. (57.3%) of respondents in the sample were nurses. Most of study sample (56%) were females with 1-5 years of experience (58.7 %), the majority of respondents had taken courses on how to needles use and disposal (65%), also found that the majority of workers (60.7%) were exposed to needles and most injuries (39.3%) occurred in the patient’s room / bedside. (25.8%) during the injection and (30.3%) by used single needle and it also found that (31.4%) the design of the sharp tool was safe. Most injuries (31.7%) occurred during the use of the sharp tool and most workers (74.6%) received the hepatitis virus vaccine. The study concluded that a relatively more than a half of Health care workers are exposed to needle sticks, and this is one of the most common causes that lead to the transmission of diseases such as hepatitis. Replace sharp tools with another one more safety when using. Administrative commitment through the implementation of a program to protect workers from transmitted diseases. Activating the committee for the prevention of acupuncture injuries of needles and sharp tools. Implementing awareness and training programs for their correct use. Implementation of educational program of vaccinations, as well as raising level of reporting of puncture injuries. Safe disposal of hazardous medical waste from the source (i.e. from the place of use of the tool). Provide self-protective measures such as clothing and equipment. Putting and implementing policies on correct method for using needlestick.