Abstract : Healthcare workers are critical in improving and maintaining public health, yet they face significant occupational challenges that can compromise their well-being. Workplace stress, burnout, and mental health issues such as depression, anxiety, and stress are prevalent among healthcare professionals, with far-reaching implications for their personal health, professional performance, and patient outcomes. This study aims to assess the prevalence and associated factors of depression, anxiety and stress among healthcare workers in Al-Baha government hospitals. A hospital-based cross-sectional study was conducted among 142 healthcare workers at Al Baha city from August to September 2023. Participants were enrolled using a convenient sampling technique. Data were collected using a structured electronic survey based on the Depression Anxiety Stress Scales-21. Descriptive statistics and inferential analysis were applied and a p-value of less than 0.05 was considered statistically significant. Among 142 participated healthcare workers participated, with nurses (23.9%) and physicians (16.9%) constituting the largest professional groups. High prevalence of depression, anxiety, and stress were found with 67.6%, 73.9%, and 54.2% of participants affected respectively. Factors such as age, marital status, job category, years of experience, and working hours significantly influenced mental health outcomes. Long work hours and specific job roles were associated with higher stress and anxiety levels. The study underscores the need for tailored interventions to address the mental health challenges faced by HCWs, promote supportive workplace environments, and enhance the quality of healthcare delivery in the Al-Baha region.