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Abstract : Online motorcycle taxi drivers are frequently exposed to oxidative stress originating from air pollutants. Vitamin 25(OH)D is one of the potent antioxidants in the body. Studies on the relationship between vitamin 25(OH)D concentration and oxidative stress markers in online motorcycle taxi drivers are still rare. Objective of this study was to determine the differences between oxidative stress biomarkers levels in online motorcycle taxi drivers and control subjects, and the relationship of 25(OH)D concentration and oxidative stress biomarkers in both groups. This cross-sectional study involving 70 online motorcycle taxi drivers and 70 subjects as a control, aged 20-50 years. The study was conducted from September 2022 to March 2023. Subjects meeting inclusion criteria were asked to fill in a questionnaire, then underwent examination of vital signs and anthropometric data, after which 10 mL of their venous blood was drawn for examination of 25(OH)D, 8-OHdG, and MDA. Spearman’s correlation and T-test was used with p <0.05 indicating significant difference. Mean age of online drivers was 36.4±7.9 years, whereas mean age of control subjects was 38.5±6.7 years, There was significant differences between 8-OHdG and MDA concentration at online drivers and control subjects. There was no significant correlation of 25(OH)D concentration with MDA and 8-OHdG concentrations in both groups.