There are thousands of variations of the coronavirus virus, which affects the epidemic of this disease; therefore, monitoring these variables is necessary to understand the course of the epidemic and the impact of the type of variations on the severity of the disease. Coronavirus disease is a recent viral disease that has caused a modern global health pandemic since the influenza pandemic in 1918. The study was conducted by collecting nasal swabs of 100 patients with positive samples of COVID-19 virus in cooperation with the Central Public Health Laboratory (CPHL) and Imam Ali Hospital in Baghdad. The SARS-CoV-2 Variant Typing Real-TM (Real-Time PCR kit for the qualitative identification of SARS-CoV-2 Variant mutations in the Spike protein/ Sacace Biotechnologies com./ Italy) diagnostic kit was used to determine the viral genomic information of the samples. The 100 positive Covid-19 PCR template samples' results revealed four different types of recognized variations. Unknown variants 39 made up the greatest portion of the sample (39%; 95%CI: 29.0 - 49.0), followed by 19 OMICRON variant B.1.1529 (19%; 95%CI: 12.0 - 27.0), 17 Brazilian/Gamma variant P.1, 15 South Afrian/Beta variant B.1.351, and 10 British /Alpha variant B.1.1.7. When using the SARS-CoV-2 Variant Typing real -TM diagnostic kit to determine the type of variant, we have 39 unknown variants, which is due to the occurrence of mutations in the genetic structure of this virus. These mutations will have many negative effects on the molecular and serological tests, so the study sample, which consisted of 100 samples, was positive for the PCR test.