Abstract : Urinary Tract Infections (UTIs) are among the most common bacterial infections encountered in clinical practice, and their management has become increasingly challenging due to rising antimicrobial resistance. The present study aims to analyze the prevalence and antimicrobial resistance patterns of uropathogens in patients admitted to Government General Hospital, Kadapa. This prospective study was conducted on urine samples collected from May 2024 to December 2024. The samples were processed for bacterial culture and antimicrobial susceptibility testing using the Kirby-Bauer disk diffusion method according to Clinical and Laboratory Standards Institute (CLSI) guidelines. The prevalence of different uropathogens and their resistance patterns were analyzed. A total of 307 urine samples were found positive for significant bacterial growth. Escherichia coli was the predominant isolate (51.1%), followed by Klebsiella spp. (16.3%), Coagulase-negative Staphylococcus (CONS) (22.8%), Pseudomonas aeruginosa (2.6%), and Staphylococcus aureus (2.6%). A high prevalence of multidrug resistance (MDR) was observed among the isolates. E. coli demonstrated high resistance to ampicillin (86%), cotrimoxazole (69%), and fluoroquinolones (ciprofloxacin, 57%). Klebsiella spp. showed significant resistance to beta-lactams and fluoroquinolones. Pseudomonas aeruginosa exhibited high resistance to cephalosporins and aminoglycosides but retained sensitivity to carbapenems. Staphylococcus aureus showed methicillin resistance in 62% of cases, and Candida spp. were isolated in 2.0% of cases, indicating possible fungal UTIs. The study highlights the alarming rise of antimicrobial resistance among uropathogens, limiting treatment options. Routine antimicrobial susceptibility surveillance and strict antibiotic stewardship programs are crucial to mitigating the emergence of drug-resistant infections in tertiary care settings.