Enterococcal bacteria have emerged as the main nosocomial pathogens. Virulence factors are important in enhancing the ability of Enterococcus faecalis to occur diseases in patients. Resistance genes only do not indicate the pathogenicity of bacteria and present them with virulence factors it can cause the strain to be dangerous. Ten Enterococcus faecalis were isolated from patients with vaginitis. All isolates were confirmed by biochemical tests and selective media and All isolates were completely resistant to gentamicin, 40% of isolates were resistant to chloramphenicol, and All isolates were highly susceptible to ampicillin, vancomycin, and nitrofurantoin. the virulence factors were detected by multiplex PCR. It was found that Esp and ClyA were observed in (100%), and (90%) In E. faecalis respectively. virulence factors commonly prevalent in Enterococcus faecalis isolated from vaginitis in this study.