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Abstract : Klebsiella pneumoniae belongs to the Enterobacteriaceae family and is described as a gram-negative, encapsulate, and non-motile bacterium. ESBLs can hydrolyze oxyimino cephalosporins rending third-generation cephalosporins ineffective against treatment. Due to this resistance, carbapenems became a treatment option for ESBL. Enterobacteriaceae acquire ESBL genes by mutation or horizontal transfer of plasmids, which results in oxyimino-cephalosporin resistance. The most common ESBL-encoding genes are blaCTX-M, blaTEM, blaSHV and blaOXA. This cross-sectional study was carried out in the Department of Microbiology of Santosh Medical College & Hospital, Ghaziabad in association with Govt. Medical College & super facility Hospital, Azamgarh. All clinical samples including pus, urine, sputum, blood and fluid etc. DNA was extracted from bacterial colonies using DNA extraction kits. The presence of three variants of ESBL (SHV). The DNA templates were extracted from the overnight cultures of ESBL-producing enterobacteria grown on the nutrient agar. Out of 256 K. pneumoniae isolates, distribution of ESBL screening positive K. pneumoniae isolates in various clinical samples are shown in the Graph 4. Among the isolates screened for ESBL production, 54.2% of urine sample and least were blood sample 13.6% of K. pneumoniae. Of the total 256 in K. pneumoniae, 39 (53.4%) isolates harbored blaCTX-M, 21 (28.7%) blaTEM, and 13 (17.8%) blaSHV. Patients predominantly contain blaCTX-M clusters, which could be the genesis, causing hasty changes in drug-resistance epidemiology. CTX-M-1 and CTX-M-15 are also commonly found among human pathogens suggesting a common clonal lineage and a potential ESBL dissemination source. These superbugs respond to fewer antibacterials, including colistin, piperacillin-tazobactam, carbapenems, and cefoperazone-sulbactam, which is alarming.