Pseudomonas aeruginosa keratitis is the most common eye infection, that develops very rapidly and can lead to endophthalmitis and corneal perforation. Corneal wound healing process is determined by the balance of pro-inflammatory and anti-inflammatory cytokines. Interleukin-1 alpha (IL-1α) and Transforming Growth Factor-β (TGF-β) as “master regulator” cytokines. Mesenchymal stem cells can maintain the equilibrium of these two inflammatory cytokines. The study was to analyze the effect of MSC metabolite to the expression of IL-1α and TGF-β. This study was an animal experimental study. The mice were randomly divided into 3 groups (no treatment, antibiotic and antibiotic-MSC metabolite group). Each group consisted of 6 mice. Keratitis was induced by 5 µl Pseudomonas aeruginosa suspension containing 104 CFU/ml. Isolation of limbus mesenchymal stem cells and cell culture were conducted. The Immunoreactive Score (IRS) and immunohistochemical staining were used to assess IL-1α and TGF- β expression. The mean expression of IL-1α in the antibiotic group with Immunoreactive Score (IRS) was 5.03 ± 3.03, while in the antibiotic + MSC metabolite group it was 4.5 ± 2.51. TGF-β expression in the antibiotic group obtained a mean score of 4.26 ± 2.31, while in the combination group of antibiotics and MSC metabolites the mean score was 2.8 ± 1.30. There was a decrease of the IL-1α and TGF- β expression in the group that was added to the MSC metabolite. The Independent T-Test showed that there was no significant difference between the antibiotic group and the antibiotic + MSC metabolite group p>0.05 (p=0.673 in IL-1α and p = 0.143 in TGF-β expression). Mesenchymal stem cell (MSC) metabolite is a potential cell therapy for corneal wound healing of Pseudomonas aeruginosa keratitis, but further studies are still needed with more sample.