A peri-expiremental study was carried out at Alkarkh General Hospital in Baghdad city to assess the level of melatonin after application of topical tretinoin for the management of skin wrinkles, age spots and dyschromia for women from December 2020 to September 2021. Aimed to assess and correlate the level of melatonin as an antioxidant agent and its role in reduction of skin wrinkles after topical application of tretinoin. Forty five women their ages were ranged from (35-55 years) involved in this study. Patients were assessed for melatonin level by collecting blood samples before and after treatment. Results showed that serum melatonin increased after applied topical tretinoin twice daily for three months. Application of topical tretinoin for aged facial skin for a limited time period can significantly reduce skin wrinkles by increasing serum melatonin levels as an antioxidant agent.