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Abstract : Enterococcus faecalis is a bacteria that often causes secondary infections within the root canals therefore, the selection of appropriate irrigation materials is required at the treatment of root canals. Moringa leaf extract (Moring aolifera) is a natural ingredients that contains secondary metabolites such as, alkaloids, tannins, saponins and flavonoids, which is beneficial as an antibacterial substance. The objective of this research is to discover if inhibition effect Moringa leaf extract (Moringa oleifera) with concentration of 25%, 50%, 75%, and 100% is able to inhibit the growth of Enterococcus faecalis bacteria through in vitro. This research is a laboratory experimental research with post test only control group design. The Moringa leaf extract was obtained by using maceration technique with 100% in which Moringa leaf extract was liquefied with DMSO to achieve 25%, 50%, and 75% concentration and as control treatments. This research was conducted with 5 controls treatments and each control treatments were done in 6 times repetitions. The inhibition effect was conducted by using disc diffusion method. A clean disc paper was dipped into each concentration for 15 minutes, and transferred into MHA media that has been covered with a suspension of Enterococcus faecalis bacteria, and then incubated at 37 oC for 24- 48 hours to observe the inhibition effect. Result: According to the research result of Moringa leaf extract (Moringa oleifera) at 25%, 50%, 75%, 100 % concentration and DMSO (control treatments), it does not generate inhibition effect towards the growth of Enterococcus faecalis bacteria. Moringa leaf extract (Moringa oleifera) with concentration of 25%, 50%, 75%, and 100% by using disc diffusion method does not generate inhibition effect towards the growth of Enterococcus faecalis bacteria.