Mechanical ventilation (MV) uses in neonates primarily aim to assist or replace spontaneous breathing completely, but prolonged use of MV has been associated with various injuries and increased mortality rates. Therefore, extubation must be planned as early as possible, but due to the lack of standardized guidelines, the decision on when to extubate is difficult to determine. The aim of this study is to find the factor that becomes a predictor of the successfulness of extubation among neonates. This study is an observational cross-sectional study was conducted at the neonatal intensive care units (NICU) in the Government General Hospital Haji Adam Malik in February-May 2022. The sample were neonates treated with MV. The sample will be divided into two groups, consisting of successful extubation and failed extubation group. Data were collected and analyzed, and a p-value <0.05 is considered a significant result. From 46 neonates, the mean age of the sample was 10.22 days with males predominant (56.5%). Successful extubation was found in 40 neonates (87%), and extubation failure only occur in 6 neonates (13%). There were statistical differences in FiO2, MAP, PIP, neutrophils, procalcitonin, pH, and SaO2 between the successfully extubated neonates when compared to fail extubated neonates (p < 0.05). The conclusion of this study is FiO2, PIP, MAP, neutrophils value, procalcitonin value, blood pH, and SaO2 can be considered reliable factors that can predict the likeliness of successful extubation.