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Abstract : The main problem that often occurs after tooth extraction is bone damage. The alveolar bone will resorb by 40% to 60% after tooth extraction, making it difficult to rehabilitate dentures. Golden sea cucumber is rich in growth factors that have the potential to repair damaged cells after tooth extraction. The protein content of golden sea cucumber reaches up to 82%. This protein content is very important to function for bone remodeling so that alveolar bone preservation can be maintained. This study aims to analyze the content of collagen, chondroitin sulfate, and glucosamine content in golden sea cucumbers. The type of research used is an experimental laboratory by extracting sea cucumber and calculating yield results to obtain the percentage of collagen content and conducting analysis using the High-Performance Liquid Chromatography (HPLC) system to obtain levels of chondroitin sulfate content and spectrophotometry to obtain glucosamine content levels. The content of collagen in Stichopus hermanii is 0.159%. The chondroitin sulfate content of 91.91 (mg/g) or equal to 9,191%, and the rate of glucosamine content in gold braids is 186 (mg/g) or 0.186%. Golden sea cucumber originating from the Selayar islands contains collagen, chondroitin sulfate, and glucosamine which can be used as preservation material for alveolar bones.