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Abstract : This study aimed to evaluate the remineralization potential of sodium monofluorophosphate (NovaMin®) compared to a commonly used standard toothpaste (Signal®). Thirteen enamel blocks of primary canines were divided randomly into two groups: Group 1 (n = 15; Experimental), NovaMin® toothpaste group, and Group 2: (n = 15; Control), Signal® toothpaste. The samples were initially evaluated for baseline surface microhardness; later on, these samples were placed in the demineralizing solution for 69 h, and post-demineralization surface microhardness was measured. Thereafter, the samples were stored in the toothpaste for 3 minutes twice daily for 15 days, and surface microhardness was recorded. The surface microhardness was evaluated using a Vickers microhardness tester. Statistical analysis was done using dependent and independent sample tests. The results showed that there is a statistically significant difference in the mean enamel microhardness after remineralization (p=0.000) between the NovaMin (399.99) and Signal (300.62) groups in favor of the NovaMin group. The NovaMin toothpaste showed promising potential to promote remineralization of demineralized human enamel.