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Abstract : Anemia is a common condition during pregnancy that is characterized by low levels of hemoglobin, which can have negative effects on both the mother and the baby. Previous studies have shown that iron supplementation can improve hemoglobin levels, but in this study wanted to evaluate the effect on the viability of gut Bifidobacterium sp., Lactobacillus sp., Clostridium sp., and Escherichia coli in anemia pregnant women from Moringa honey. This randomized controlled trial (RCT) involved pregnant anemia (20-28 weeks) women who were given Moringa honey as the intervention group and healthy honey as the control group for eight weeks. Participants consumed honey products during the eight weeks of the intervention period. Hemoglobin was measured before and after the intervention. Stool samples were also collected at both times. DNA was extracted from fecal samples. RT PCR (real time polymerase chain reaction) was performed to determine the changes in gut Bifidobacterium sp., Lactobacillus Sp., Clostridium Sp., and Escherichia coli amount after the intervention. Twenty-nine individuals, including 15 from the intervention group and 14 from the control group, completed the study. However, Bifidobacterium sp., Clostridium sp., and Escherichia coli levels in anemic pregnant women were not substantially increased in the invention group (p=0.223, p=0.074, and p=0.314, respectively. In addition, we observed a larger rise in Lactobacillus sp. in the intervention compared to the control group (p=0.04). Moringa honey affected increasing gut Lactobacillus sp., but not on Bifidobacterium sp., Clostridium sp., and Escherichia coli in anemic pregnant women.