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Abstract : Integrated management of neonatal and childhood illnesses (IMNCI) is a number of guidelines build ‎up by the World Health Organization (WHO) in 1992 to improve the assessment, classification, and ‎the individual management of the common causes of death among children ‎under the age of five [1].‎ The development of the IMCI strategy was based on three main components, improving the performance of health care providers, enhancing the performance and management structure of the health system and strengthening family and community practices [2]. To evaluate the impact of educational ‎program ‎regarding the integrated management of ‎neonatal and childhood illness ‎on nurses ‎practices‎. A quantitative study (quasi-experimental design) carried out for achieving the objectives of present study, by application of pretest- posttest approach for both study and control groups about nurses working in IMNCI units from 20th September 2020 to 25th April 2022. The study is conducted in the primary health care centers of the four health care sectors in Holy Karbala. Non probability sample (purposive sample) from the total population who meet the criteria of the study during the study interval. A total of (60) nurses selected for the study, (30) participant for study group and (30) for control group. The mean age of study group was 33.5 ± 8.1 years and that of control was 32.8 ± 8.8 years, while males were dominant in study group and controls represented 63.3% and 70%, respectively. Vast majority of the participants, 90%, in the study group and 80% of controls were married. In regard educational achievement (66.7%) and (76.7%) of (study group and control) had achieved nursing institute level of education. Comparison of overall mean practice scores of study group and controls for all domains revealed no significant difference in mean scores before education program (pretest) between both groups, (P>0.05), a highly significant differences in means score at post-test (1) and post-test (2), at (P<0.001). Within each group, it had been found that the change in overall Practice scores increased significantly in the study group where the overall mean score for all domains increased from 2.21 ± 0.20 before education program to 2.76 ± 0.09 at posttest 1 and almost maintained to 2.75 ± 0.11 at posttest 2, with a mean difference of 0.54 and a percentage change of 24.4%, the change was highly significant, (P<0.001). In control group, the change was statistically insignificant where the mean score before education was 2.29 ± 0.17, at posttest 1 was 2.30 ± 0.16 and at posttest 2 it was 2.31 ± 0.16 with a mean difference of only 0.02 and the percentage change was 0.9%, (P>0.05). Significant improvement appear clearly among the interventional group member through the result of their pre-test and post-test, which explain the effectiveness of the reinforcement educational program session content on the nurses practices.