Abstract : To analyze the effect of an evidence-based insulin injection technique education program on insulin injection technique practice and HbA1c values. A quasi-experimental with non-equivalent control group pretest-postest approach. Sixty respondents were applied and selected by purposive sampling. The practice of insulin injection technique was measured using the Worldwide Injection Techniques Questionnaire. Data were analyzed using paired sample t-test and independent sample t-test. The evidence-based insulin injection technique education program had an effect on the practice of insulin injection technique (p=0.004) and had an effect on the HbA1c value (p=0.000). The education program for providing evidence-based practice-based insulin injection techniques affects the HbA1c value of diabetes mellitus type 2 patients, so it is recommended for the hospital to hold an educational program for providing evidence-based practice-based insulin injection techniques so that the glycemic levels of diabetes mellitus type 2 patients can be controlled.