Abstract : The respiratory tract has sites which are potentially inaccessible to microbes. But, microbes when enter, they caused Respiratory Tract Infections, which in turn become more resistant for treatment due to improper use of antibiotics, in addition they prescribed by unspecialized persons. This led to create microbes which are different from the original microbes by gaining new strategies for infection and persistence, antibiotic resistance, different virulence factors, various phenotypes and adaptive changes to cause host damage and disease. The generators produce harmful exhaust emissions such as; carbon monoxide, nitrogen oxides, hydrocarbons, particulate matter and sulfur dioxide. These exhaust emissions are harmful and can not be ignored especially for all the living creatures and surrounding environment. 184 Sputum samples from both workers and non-workers in electric generators who suspected had lower respiratory tract infection were collected. All samples were cultured and identification of bacteria and antimicrobial sensitivity tests were done by using a Vitek 2 system. Detection of some virulence factors was done. Out of 184 samples, 27 samples (14.67 %) produced a significant growth, The predominant bacteria was Klebsiella spp. The highest No. of Resistant bacterial isolates of Workers in electrical generators were for: Ampicillin, Cefazolin and Nitrofurantion. There were different levels of antibiotic resistance in both groups, in addition to different virulence factors presence (e.g. capsule, Lipase, protease and ESBLs, Efflux pump and string test). Statically, there were significant difference between both groups at (p<0.05) for biofilm formation.