Nursing care for comatose patients admitted to critical care units requires carrying out essential care to enable patients to perform daily life tasks and prevent complications. The comatose patients are entirely dependent on critical care nurses for recovery, and it is the nurse's responsibility to always think critically before intervening; thus, critical care nurses working in these areas must have sufficient knowledge to assess and intervene. This study aimed to assess the critical care nurses' knowledge regarding nursing care for comatose patients. A descriptive study (cross-sectional) was conducted to accomplish the aim of this study from the period between 19th September 2021 up to 6th July 2022. A non-probability (purposive) sample consisted of (178) critical care nurses who worked in the CCU and ICU in the six hospitals at Babylon Governorate was selected. The results of this study showed that the highest percentage (74.2%) of critical care nurses were aged ranging between 22 and 29 years old, more than half (51.1%) were females nurses, and (61.2%) of them were married, in addition, more than half of the participants (53.9%) were graduated from colleges of nursing. Moreover, most of the study sample (66.9%) had (1-5) years of experience in nursing. In relation to the work unit, more than half of critical care nurses (59%) worked in ICU, while most of the participants (62.9%) had (1-3) years of experience in their current workplace, and more than half of them (53.4%) were worked at evening shift and (55.1%) of them did not have any training courses about nursing care for comatose patients. The overall findings of critical care nurses' knowledge demonstrated that (78.7%) of them expressed a moderate level of knowledge related to nursing care for comatose patients. The overall findings illustrated that the majority of critical care nurses expressed a moderate level of knowledge regarding nursing care for comatose patients.