About the Journal

Download [This article belongs to Volume - 63, Issue - 09]

Abstract : Evaluation of oocyte quality is one of the most important goals of embryologists in assisted reproductive technologies, due to its importance in successful fertilization and embryonic development. Females undergoing in vitro fertilization are exposed to massive systemic oxidative stress due to the ovarian stimulation protocols used, which negatively affects oocyte maturation and embryonic development. Glutathione peroxidase is one of the most important endogenous antioxidants purported to defend against oxidative stress by modifying free radical electrons, and therefore it is necessary to investigate the effect of its blood levels on oocyte maturation and the embryo quality in women undergoing in vitro fertilization treatment. An observational, cross-sectional study was conducted on 60 females undergoing IVF treatment by pituitary down-regulation with GnRH agonist. Glutathione peroxidase-1 levels in serum and follicular fluid were determined using an ELISA kit. The maturity of the oocyte nucleus as well as the development of the resulting embryos after sperm injection are evaluated by IVF specialists. Grade Ι embryos resulting from the injection of high-quality oocytes are what the doctor seeks to obtain to return these embryos to the uterus. We investigated the relationship between serum glutathione peroxidase levels with the number and quality of MΠ oocytes and Grade Ι embryos using Statistical Package for Social Sciences version 23. We found that the relationship between GPX activity in the blood (measured in µU/ml) and the number of mature oocytes (MΠ), maturation rate, fertilization rate, and the number of Grade Ι embryos were found to be statistically non-significant, with a p-value of 0.709, 0.327,0.720, 0.385, respectively. The GPX activity measured in the blood was not associated with any IVF/ICSI indicators. This suggests that further comprehensive research is needed in this area.