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Abstract : Although irrigation is one of the most important stages during root canal treatment, it is not possible to guarantee the entry of irrigants to all the areas in the root canal, especially in the apical third. Therefore, the activation of irrigants can stimulate and help the irrigants to access hard-to-reach places within the root canal system. Therefore, this study aimed to evaluate the penetration depth between sodium hypochlorite (NaOCl) gel and solutions into dentinal tubules after ultrasonic irrigation (PUI) and mechanical activation with the XP- endo Finisher instrument. The study sample consisted of 40 single-canal teeth, which were randomly divided into the following four equal groups according to the activation method used and Sodium hypochlorite type: group 1 (n = 10), Ultrasonic activation for Sodium hypochlorite solution (USS), group 2 (n = 10), Ultrasonic activation for Sodium hypochlorite gel (USG), group 3 (n = 10) XP-endo Finisher activation for Sodium hypochlorite solution (XPS), and group 4 (n = 10) XP-endo Finisher activation for Sodium hypochlorite Gel (XPG). Afterward, dental crowns were cut to standardize the working length by 16 mm and were then prepared using the ProTaper Next system up to size F3. Moreover, the irrigant was activated for each group separately, and the teeth were sectioned horizontally for coronal, middle, and apical thirds. The penetration depth of irrigants into dentinal tubules was measured in micrometers by viewing the bleached crystal violet under a stereomicroscope at ×20 magnification., images have been transferred to the Adobe Photoshop program, and dye penetration depths were measured. Data were analyzed using the One-way Anova and Bonferroni tests. PDSH in USS was greater than USG (p = 0.015), PDSH in USG was also lower than XPS (p = 0.001) and XPG (p = 0.001) in the coronal third. PDSH in XPS was greater than USG (p = 0.0102) in the middle third. PDSH in USS was greater than USG (p = 0.000) and XPG (p = 0.004), PDSH in USG was lower than XPS (p = 0.000) and XPG (p = 0.040) in the apical third. sodium hypochlorite solution is superior to sodium hypochlorite gel in the depth of its penetration into the dentinal canals when activated by ultrasonic, and the XP-endo Finisher activation was similar in irrigant penetration within the root canal system, especially in the apical third, when compared with ultrasonic activation. However, the sodium hypochlorite solution was similar to sodium hypochlorite gel in the depth of its penetration into the dentinal canals when activated by XP-endo Finisher activation in the apical third.