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Abstract : Blunt force injuries is the most common type of violence in physical abuse. There were several cases of physical abuse due to blunt force where the victim who was traumatized by blunt force did not have any damage to the skin. Factors that affect the occurrence of trauma without a damage to the skin include the location of contact between the trauma and the body region, pressure (force), the time lag between the victim experiencing trauma and the time to see a doctor, the color of a person's skin, blood pressure, and the thickness of skin tissue. This can be an obstacle for doctors to find physical evidence due to trauma to be written into the medico-legal reports. Blunt trauma that occurs in a person's body will trigger pro-inflammatory mediators such as Interleukin 1-β (IL-1β), Interleukin-6 (IL-6) and Tumor Necrosis Factor-α (TNF-α). This study is an experimental study using animal models. The samples were divided into the control group and the group which the blood serum was taken 1 and 6 hours after being blunt traumatized. The results show there was no significant difference in IL-1β (p=0.161) and TNF-α (p=0.678) levels and there was a significant difference in IL-6 levels (p=0.004) between the control and intervention groups. There was a significant difference on IL-6 cytokine levels between the control group and the intervention groups. The results indicate that pro-inflammatory cytokine IL-6 has the potential to become new examination procedures in cases of trauma without damage to the skin.