Abstract : HIV infection is associated with progressive immunosuppression, increasing susceptibility to opportunistic pathogens such as Candida. and Staphylococci. Colonization by these organisms may precede clinical disease and vary with demographic factors and CD4 counts. The present study aimed to determine the prevalence and distribution of Candida and Staphylococcus colonization among HIV-positive individuals and analyze their association with age, gender, and CD4 counts. A cross-sectional study was conducted among 447 HIV-positive individuals attending a tertiary care ART center. Demographic data were recorded, and oral and nasal swabs were collected. Candida isolates were identified by culture, germ tube test, and CHROM agar, while Staphylococcus was identified using standard biochemical tests. CD4 counts were retrieved from medical records. Data were analyzed using the Chi-square test, with p < 0.05 considered statistically significant. Of 447 participants, 253 (56.6%) were male and 194 (43.4%) were female, with most (71.8%) aged 30–60 years. Candida colonization was observed in 128 (28.6%) and Staphylococcus in 76 (17.0%) individuals, with no significant gender association (p > 0.05). C. albicans (60.9%) was the predominant species, followed by C. tropicalis (17.2%), C. glabrata (11.7%), and C. krusei (10.2%). Colonization was highest in participants with CD4 counts >500 cells/µL (84.6%), but this was not statistically significant (p = 0.483). Candida and Staphylococcus colonization in HIV-positive individuals showed no significant correlation with gender or CD4 count. Routine screening, species-level identification, and further multicentric studies incorporating molecular methods and antifungal susceptibility testing are warranted.