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Abstract : Pseudomonas aeruginosa often causes severe nosocomial infections that is resistant to several antibiotic classes in hospital setting. Multidrug resistant (MDR) Pseudomonas aeruginosa infection increases morbidity and mortality as well as hospital expenses. The study was to analyze the antibacterial activity of Centella asiatica against MDR Pseudomonas aeruginosa. It was a laboratory experimental study. C. asiatica was extracted by sonication method. Phytochemical Test was conducted against flavonoid, phenolic acid, alkaloid, tannin, steroid, triterpenoid and saponin. The test groups divided into 5 groups (no treatment, Colistin 10 µg, 1000, 3000 and 5000 ppm of Centella asiatica extract) and each group consisted of 4 replications. The antibacterial activity had been tested by Kirby Bauer disc diffusion method. The diameter of inhibition zone was measured and calculated by formula. The best results of zone of inhibition of concentration is a 5000 ppm of Centella asiatica extract (means 3.125 mm). The highest inhibition zone of a 5000 ppm of Centella asiatica extract was 5 mm (SD ± 1,65) whereas zone of inhibition of Colistin 10 µg as a potent antibacterial therapy against MDR Pseudomonas aeruginosa was 8 mm. The ANOVA Test showed a significance difference between zone of inhibition for each 1000 ppm, 3000 ppm, 5000 ppm and Colistin 10 µg as antibacterial therapy for MDR Pseudomonas aeruginosa (p < 0.05). Centella asiatica has antibacterial activity against MDR Pseudomonas aeruginosa as indicated by the phenolic acid and tannin compounds. The higher concentration of Centella asiatica extract the higher antibacterial activity against MDR Pseudomonas aeruginosa.