Self-injury is an activity that is concealed by the person who engages in it. According to previous research, 32.1% of nursing students had self-injury behavior. Self-injury behavior is directly related to suicidal ideation. Personality factors can assist in the selection of interventions to treat self-injury behavior, so it becomes important to examine the relationship between personality and self-injury. This study aims to identify the relationship between personality factors, specifically the Big Five Personality, and self-injury behavior. The research design used a descriptive correlational design. The population of this study was undergraduate students in the Faculty of Nursing, with a sample of 438 students. The instruments used are the Big Five Inventory (BFI) and the Deliberate Self-Harm Inventory (DSHI). The data was analyzed using descriptive statistical methods and the Rank Spearman. The results showed that there was a positive significant relationship between neuroticism and self-injury behavior, while between agreeableness and conscientiousness and self-injury behavior there was a negative significant relationship. The higher an extraversion score, the greater the risk of self-injury; however, the higher agreeableness and conscientiousness scores, the lower the risk of self-injury.