The use of plants as a source of natural antioxidants has been widely studied. One of the plants that have the potential as natural antioxidants is beligo (Benincasa hispida (Thunb.) Cogn.). The part of the beligo fruit that is commonly used is the seeds. Empirically beligo has been widely used by Indonesian people as a medicinal plant. Indonesian people use beligo as a fever reducer, pain and typhoid treatment, presumably based on its phenolic content. Phenolics have a role in antioxidant activity. Ethanol 70% is a solvent that has been proven to be widely used to extract total plant phenolic compounds. This study aims to determine the phytochemical content, phenolic content and antioxidant potential of ethanol 70% extract of beligo (Benincasa hispida (Thunb.) Cogn.) seeds. Samples were extracted by maceration using ethanol 70%. Qualitative analysis of phytochemical compounds using specific reagents, analysis of total phenolic content using the Folin–Ciocalteu method at a wavelength of 764 and an antioxidant activity test using the DPPH method at a wavelength of 515 nm. Ethanol extract of Beligo (Benincasa hispida (Thunb.) Cogn. seeds positive contains alkaloids, flavonoids, phenolics and saponins. The results of the analysis of total phenolic content and antioxidant activity test showed that ethanol 70% extract had a total phenolic content of 56.8 mg GAE/g extract with an IC50 value of 46,037 ppm. Ethanol 70% extract has a very strong category of antioxidant activity so it has the potential to be used in the treatment of antibacterial, cardiovascular and anti-inflammatory diseases.