Abstract : Imidazole drugs are extensively used in the treatment of various fungal infections. Its derivatives have many biological activities. Synthesis and evaluation of antimicrobial activity of a novel derivative of imidazole has been studied. Ligand of 4-((4,5-diphenyl-1H-imidazole-2-yl) diazenyl) benzoic acid was prepared by reaction of 4,5-diphenyl imidazole with the diazonium dye. Its physical and chemical characteristics have been identified. An antimicrobial action was performed against bacteria and fungi using a disk diffusion method. The sold red ligand (C22H16N4O2) has a melting point of 210°C, a 425 nm UV spectrum and variable IR spectra. The ligand showed good antibacterial effect without antifungal activity. Gram-positive bacteria were more susceptible to the ligand than Gram-negative bacteria. The fungus Candida utilis was the only fungal species showing susceptibility to the ligand in MIC 7 mg/ml. The novel synthesized 4-((4,5-diphenyl-1H-imidazole-2-yl) diazenyl) benzoic acid has broad-spectrum antibacterial activities with less antifungal action. Gram-positive bacteria are most susceptible to the prepared imidazole derivative. The ligand may be considered a novel antibacterial agent and needs further pharmaceutical studies to confirm its ability to use for humans.