Abstract :
Obesity is an accumulation of abnormal or excessive fat that poses a risk to health. According to Asia pacific criteria, obesity in adults is defined as BMI ≥25. The gut microbiota has recently been put forward as an environmental factor responsible for weight gain and energy metabolism disorders that cause obesity. Polyphenols such as anthocyanins found in black rice (Oryza sativa L) are expected to play a role in the homeostatic gut microbiota in suppressing obesity and other metabolic diseases. This study aims to determine the analysis of the intestinal microbiota of Phylum Firmicutes, Bacteroidetes, Actinobacteria, and protobacteria in Obese Subjects who were consumed with black Rice extract through feces examination. This type of research is a Quasi Experimental laboratory with a double blind Randomized Control Trial (RCT) design. The research design used was a design with a control group (The Pre and Post Test Control Group Design). The sample was Obese Patients aged 18-25 years. The study was divided into 3 groups. Where Group 1 Ideal body weight was given plaebo, group 2 Obesity was given placebo, and group 3 Obesity was given Black Rice Extract 5.6 gr/dl. The data is presented descriptively and the Test differs paired t-test. The results of the study found in groups 1 and 2 there was a difference between the pre and post tests but not significant. Meanwhile, in group 3, there were significant differences in the decrease in the post-test value of the Phylum Firmicutes Genus Clostridium Leptum Microbiota and the comparison with the total bacteria in the post test. Conclusion: Black Rice Extract contains high Anthocyanins so that it can be used as a prebiotic ingredient for the intestinal microbiota in obese subjects. This was proven that obese patients who were given black rice extract for 4 weeks experienced a significant decrease in the post test of the Closridium Leptum Microbiota which is a gram-positive Pathogenic Bacteria.