Laryngopharyngeal reflux (LPR) is the backflow of gastric and or duodenal fluid into the larynx, pharynx, trachea, and bronchi. The prevalence of LPR is difficult to determine due to the limited gold standard and the large variety of LPR symptoms. Damage can occur due to the decrease in pH value and also because of exposure to harmful enzymes in reflux, including pepsin, bile acid, and trypsin. This study is an analytic observational study with a case-control design. The study was conducted in the ORL-HNS Department of Dr. M. Djamil Hospital, Padang, West Sumatra, Indonesia. The total sample size was 44 people. We enrolled 22 healthy subjects as the control group and 22 patients suspected of having LPR. LPR patients are more common in women than in men, with 12 women and 10 men. Pepsin levels in saliva in the LPR group were higher than those in the non-LPR group. For pepsin, the LPR group had a mean of 20.11±9.76 ng/mL and a healthy control with mean of 15.77 ±7.65 ng/mL. Trypsin levels in saliva in the LPR were 103.15±47.69 µg/mL, In the healthy group, the mean was 82.99 ±39.62 µg/mL. For Bile acid in the LPR group, in LPR, the mean of pepsin is 25.08±7.67µM, meanwhile in healthy group, the mean was 18.99±8.26 µM. There is statistically significant in the incidence of LPR with the bile acids (p = 0.015) based on ANOVA and Logistic regression test. Our study confirmed that bile acids in saliva play a major role in diagnosing LPR.