Stunting is the biggest threat to human quality of life in the future because it can inhibit physical growth, inhibit children's brain growth (cognitive), reduce the quality of learning to decrease productivity in adulthood and increase the threat of non-communicable diseases. Stunting is caused by low nutritional intake and recurring illnesses which are reinforced by an unhealthy environment. This study aims to analyze the determinants of stunting in toddlers at 12 loci in Ambon City. This type of research is quantitative research with analytic observational methods, namely research that is directed to explain a situation or situation. Sampling was carried out through two stages, namely the purposive sampling method and the simple random sampling method with a total sample of 371 toddlers. Data processing is done using SPSS. The results showed that there was a significant relationship between history of breastfeeding, number of calories, parents' income and the condition of the drinking water source with the incidence of stunting, while the sanitation variable was not related to the incidence of stunting. Suggestions for the Puskesmas, namely that the Puskesmas is expected to conduct counseling and health education regarding stunting in the community, for the community, namely parents of toddlers, they must pay more attention to the number of calories/day in the form of macronutrients and micronutrients and in the first 6 months of life are obliged to provide exclusive breastfeeding, the condition of water sources drink so as to prevent stunting in toddlers. As for the advice for the Health Service, this index is expected to be considered and utilized as a measuring tool in assisting health workers.