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Abstract : This study aimed to evaluate the remineralization potential BioMin-F compared to a commonly used standard toothpaste (Signal®) in primary teeth. Thirteen enamel blocks of primary canines were divided randomly into two groups: Group 1 (n = 15; Experimental), BioMin-F toothpaste group, and Group 2: (n = 15; Control), Signal toothpaste. The samples were initially evaluated for baseline surface microhardness; later on, these samples were placed in the demineralizing solution for 69 h, and post-demineralization surface microhardness was measured. Thereafter, the samples were stored in the toothpaste for 3 minutes twice daily for 15 days, and surface microhardness was recorded. The surface microhardness was evaluated using a Vickers microhardness tester. Statistical analysis was done using dependent and independent sample tests. The results showed that there is a statistically significant difference in the mean enamel microhardness after remineralization (p=0.000) between the BioMin-F (353.62) and Signal (300.62) groups in favor of the BioMin-F group. The BioMin-F toothpaste showed promising potential to promote remineralization of demineralized primary human enamel.