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Abstract : Chronic suppurative otitis media (CSOM) is a commonly encountered middle ear infection. Empirical use of anti-microbials for treatment of CSOM although effective, but at times, may contribute to development of anti-microbial resistance. The present study was conducted to know the antibiogram of aerobic bacteria isolated from CSOM. This was a cross-sectional, prospective study conducted in North Karnataka region from October 2021 to May 2022. The sample collection, transportation, identification of organisms and antibiogram was done using standard guidelines. Out of 124 samples, microbial growth was observed in 109 cases (65%), while no growth was seen 15 (28%) cases. Pseudomonas aeruginosa was the most common organism isolated (45.7%), followed by Staphylococcus aureus (32.5%). Highest resistance of Staphylococcus aureus was seen with Ampicillin (54.7%), Ciprofloxacin (52.3%) and Erythromycin (52.3%). 100% sensitivity was seen with Teicoplanin, Vancomycin and Linezolid. Highest resistance of Pseudomonas aeruginosa was seen with Ciprofloxacin (62.7%), Levofloxacin (92.3%), Cefpodoxime (49.1%) and Gentamicin (35.5%). No antibiotic was 100% sensitive to Pseudomonas aeruginosa. Staphylococcus aureus, Pseudomonas aeruginosa and other Gram-negative organisms causing CSOM showed resistance to commonly used antibiotics. Every hospital is different and so are its infections. Hence, antibiogram studies should be conducted at regular intervals to identify the changing trends in resistance to antibiotics. Based on the local antibiogram studies, all hospitals should have antibiotic policy which should be periodically updated so as to prevent indiscriminate use of antibiotics, complications of CSOM and emergence of multi drug resistant organisms.